real-time rpa (exo-rpa)  (GraphPad Software Inc)

Journal: Biosensors & Bioelectronics

Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

doi: 10.1016/j.bios.2020.112592

Figure Lengend Snippet: Rapid nucleic acid extraction for pathogen detection in oral samples.

Article Snippet: Leaf and root , Candidatus Phytoplasma mali , Chemical lysis in nylon mesh bags (BIOREBA, Switzerland) , , Real-time RPA using lyophilized TwistAmp RPA kit (TwistDX, UK) , 10 gene copies/reaction , 30 min (sample-to-answer) , , , .

Techniques: Extraction, Lysis, Amplification, Virus, Membrane, DNA Extraction, Quantitative RT-PCR, RNA Extraction, Sample Prep, Magnetic Beads

Journal: Biosensors & Bioelectronics

Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

doi: 10.1016/j.bios.2020.112592

Figure Lengend Snippet: Rapid nucleic acid isolation systems for pathogen detection in urine.

Article Snippet: Leaf and root , Candidatus Phytoplasma mali , Chemical lysis in nylon mesh bags (BIOREBA, Switzerland) , , Real-time RPA using lyophilized TwistAmp RPA kit (TwistDX, UK) , 10 gene copies/reaction , 30 min (sample-to-answer) , , , .

Techniques: Isolation, Lysis, Extraction, Amplification, Polymer, Real-time Polymerase Chain Reaction, Sample Prep, Virus, Modification, RNA Extraction, DNA Extraction, Transferring, Membrane

Journal: Biosensors & Bioelectronics

Article Title: Advances in point-of-care nucleic acid extraction technologies for rapid diagnosis of human and plant diseases

doi: 10.1016/j.bios.2020.112592

Figure Lengend Snippet: Rapid nucleic acid extraction methods for plant pathogen detection.

Article Snippet: Leaf and root , Candidatus Phytoplasma mali , Chemical lysis in nylon mesh bags (BIOREBA, Switzerland) , , Real-time RPA using lyophilized TwistAmp RPA kit (TwistDX, UK) , 10 gene copies/reaction , 30 min (sample-to-answer) , , , .

Techniques: Extraction, Lysis, Amplification, Sample Prep, DNA Extraction, Real-time Polymerase Chain Reaction, Virus

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Determination of the CPV-2 LFS RPA reaction time. The CPV LFS RPA assay was performed by incubating the reaction tubes in a closed fist for different times as shown. The test line was visible when the amplification time is longer than 10 min, and there was no significant difference in visibility between the 15 min and 20 min incubation time.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques: Amplification, Incubation

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Analytical specificity of the CPV-2 LFS RPA assay. Reactions tubes were incubated in a closed fist for 15 min using 10 ng of viral DNA or cDNA as template. The results showed LFS RPA only amplified CPV-2a, CPV-2b and CPV-2c DNA but not the other viruses tested. CPV-2a: canine parvovirus type 2a; CPV-2b: canine parvovirus type 2b; CPV-2c: canine parvovirus type 2c; CDV: canine distemper virus; CCoV: canine coronavirus; CPIV: canine parainfluenza virus; PRV: pseudorabies virus.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques: Incubation, Amplification

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Analytical sensitivity of the CPV-2 LFS RPA assay. The sensitivity of the CPV LFS RPA assay was performed using a dilution range of 1.0 × 10 6 –1.0 × 10 0 copies of the standard CPV-2a DNA. The LOD of the assay was 1.0 × 10 2 copies per reaction.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques:

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Detection of CPV-2 in the clinical samples by LFS RPA. Ten samples were selected randomly from the clinical samples. Lines 1–4 are samples that were CPV-2 DNA-positive by the LFS RPA, real-time PCR and SNAP assays; lines 5–6 are samples that were CPV-2 DNA-positive by the LFS RPA and real-time PCR assays but negative by the SNAP assay; and lines 7–10 are samples that were CPV-2 DNA-negative by all three assays.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques: Real-time Polymerase Chain Reaction

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Comparison of CPV-2 LFS RPA assay with real-time PCR and SNAP assays on the clinical canine fecal samples.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques: Real-time Polymerase Chain Reaction

Journal: Molecular and Cellular Probes

Article Title: Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2

doi: 10.1016/j.mcp.2018.04.004

Figure Lengend Snippet: Comparison of CPV-2 detection results using DNA extracted by a commercial kit or by boiling. The developed CPV-2 LFS RPA assay performed well using the DNA extracted by simply boiling. Lines 1–5 show samples that were CPV-2 DNA-positive by real-time PCR with different Ct values. a, DNA extracted by the commercial kit; b, DNA extracted by boiling.

Article Snippet: Our laboratory had developed a real-time RPA assay based on an exo probe for the rapid detection of CPV-2, although the assay still depended on a specialized instrument, Genie III (OptiGene, West Sussex, UK) [ ].

Techniques: Real-time Polymerase Chain Reaction

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Primers and probes used in CaPV real-time RPA and CaPV RPA LFD assay

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: Sequencing

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: The specificity of CaPV real-time RPA assay and CaPV RPA LFD assay

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques:

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Optimal primers and probe combinations of CaPV real-time RPA assay. Three forward primers (CaPV Fe1 to CaPV Fe3), three reverse primers (CaPV Re1 to CaPV Re3) and one probe (CaPV Pe) were used to select the best combination. NC represents negative control

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: Negative Control

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Sensitivity of real-time RPA assay ( a ) Typical raw fluorescence data of CaPV real-time RPA assay using a dilution series of the pCaPV/RPA DNA. NC represents negative control; ( b ) Reproducibility of the CaPV real-time RPA assay; ( c ) The limit of detection in 95% probability based on eight replicates

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: Fluorescence, Negative Control

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Extraction efficiency of the innuPREP MP basic kit on spiked samples ( n = 24) were tested by real-time RPA assay, CaPV RPA LFD assay and CaPV real-time qPCR assay respectively

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques:

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Determination of reaction temperature and time ( a ) CaPV RPA LFD assay are performed at different temperatures as shown. b The test line is visible at 38 °C when the amplification time is longer than 10 min

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: Amplification

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Sensitivity of CaPV RPA LFD assay ( a ) The sensitivity of CaPV RPA LFD assay was performed using a dilution series of the pCaPV/RPA DNA, and NC represents negative control; ( b ) The limit of detection in 95% probability based on eight replicates of CaPV RPA LFD assay

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: Negative Control

Journal: Virology Journal

Article Title: Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

doi: 10.1186/s12985-017-0792-7

Figure Lengend Snippet: Comparison of CaPV real-time RPA assay and CaPV RPA LFD assay with real-time qPCR assay on clinical samples

Article Snippet: The threshold time of CaPV real-time RPA assay was plotted against reference DNA molecules detected and the semi-log non-regression analysis was calculated with PRISM 5.0 software (GraphPad Software, USA).

Techniques: